Overview
Type: Synthetic peptide
Form: Lyophilized Powder
5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037) is the original antigen used for immunization during Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037) generation. The blocking peptide binds and ‘blocks’ Anti-5HT7 Receptor/HTR7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1, 2. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:400).
3, 4. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody, preincubated with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 4), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:400).
4-6. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody, preincubated with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037).
Expression of 5HT7 Receptor in rat dorsal raphe nucleus (DRN)Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. 5HT7R immunoreactivity (green) appears in neuronal profiles in the lateral DRN (arrows). B. Pre-incubation of the antibody with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037), suppresses staining. Cell nuclei are stained with DAPI (blue). LDRN = lateral DRN, MDRN = medial DRN.
Expression of 5HT7 Receptor in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, shows 5HT7R immunoreactivity (green) in neuronal profiles in the hilus (vertical arrows) and in glial profiles in the outer molecular layer (OML, horizontal arrows). B. Pre-incubation of the antibody with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037), suppressed staining. Cell nuclei are stained with DAPI (blue). H = hilus, G = granule layer, OML= outer molecular layer.
Properties
Sequence
- Peptide CGEQINYGRVEK, corresponding to amino acid residues 73 - 84 of rat 5-HT7 (Accession P32305).
Accession (Uniprot) Number P32305
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
