Overview
Type: Synthetic peptide
Form: Lyophilized Powder
NMUR1 (extracellular) Blocking Peptide (#BLP-NR071) is the original antigen used for immunization during Anti-NMUR1 (extracellular) Antibody (#ANR-071) generation. The blocking peptide binds and ‘blocks’ Anti-NMUR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:500).
3-4. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).
Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human K562 myelogenous leukemia cell line lysate (lanes 3 and 7) and human NK-92 natural killer cell line lysate (lanes 4 and 8):1-4. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:400).
5-8. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).
Expression of NMUR1 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1: 300), followed by goat anti-rabbit-AlexaFluor-488. A. NMUR1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with NMUR1 (extracellular) Blocking Peptide (BLP-NR071), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)DRLMWSMVSHWTDGLR, corresponding to amino acid residues 316 - 331 of rat NMUR1 (Accession Q9JJI5).
Accession (Uniprot) Number Q9JJI5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
