Overview
IGF1 Blocking Peptide (#BLP-NT046) is the original antigen used for immunization during Anti-IGF1 Antibody (#ANT-046) generation. The blocking peptide binds and ‘blocks’ Anti-IGF1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications
Western blot analysis with Anti-IGF1 antibody:1, 4. Recombinant rat IGF-I protein (#I-200), 100 ng.
2, 5. Recombinant human IGF1, 100 ng.
3, 6. Recombinant human IGF2, 100 ng.Lanes 1-3: Anti-IGF1 Antibody (#ANT-046), (1:200).
Lanes 4-6: Anti-IGF1 Antibody, preincubated with IGF1 Blocking Peptide (BLP-NT046).Note that the antibody recognizes IGF1 (rat and human), but fails to recognize the closely related IGF2 protein.
Expression of IGF1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-IGF1 Antibody (#ANT-046), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA1 region showed IGF1 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with IGF1 Blocking Peptide (BLP-NT046), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of IGF1 in mouse parietal cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-IGF1 Antibody (#ANT-046), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. IGF1 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with IGF1 Blocking Peptide (BLP-NT046), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
- Peptide (C)NKPTGYGSSIRR, corresponding to amino acid residues 74 - 85 of rat IGF1 (Accession P08025).
