Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR139 Blocking Peptide (#BLP-GR069) is the original antigen used for immunization during Anti-GPR139 Antibody (#AGR-069) generation. The blocking peptide binds and ‘blocks’ Anti-GPR139 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1-2. Anti-GPR139 Antibody (#AGR-069), (1:800).
3-4. Anti-GPR139 Antibody, preincubated with GPR139 Blocking Peptide (BLP-GR069).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-GPR139 Antibody (#AGR-069), (1:200).
2. Anti-GPR139 Antibody, preincubated with GPR139 Blocking Peptide (BLP-GR069).
Expression of GPR139 in rat ventromedial hypothalamus (VMH).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR139 Antibody (#AGR-069), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. GPR139 immunoreactivity (green) appears in tanycyte-like processes (arrows). B. Pre-incubation of the antibody with GPR139 Blocking Peptide (BLP-GR069), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle.
Properties
Sequence
- Peptide (C)RKSNFRLRGYSTGK, corresponding to amino acid residues 204 - 217 of mouse GPR139 (Accession Q80UC8).
Accession (Uniprot) Number Q80UC8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
