Overview
Type: Synthetic peptide
Form: Lyophilized Powder
LRRTM2 (extracellular) Blocking Peptide (#BLP-NR142) is the original antigen used for immunization during Anti-LRRTM2 (extracellular) Antibody (#ANR-142) generation. The blocking peptide binds and ‘blocks’ Anti-LRRTM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-LRRTM2 (extracellular) Antibody (#ANR-142), (1:200).
2. Anti-LRRTM2 (extracellular) Antibody, preincubated with LRRTM2 (extracellular) Blocking Peptide (BLP-NR142).
Western blot analysis of mouse brain membranes:1. Anti-LRRTM2 (extracellular) Antibody (#ANR-142), (1:400).
2. Anti-LRRTM2 (extracellular) Antibody, preincubated with LRRTM2 (extracellular) Blocking Peptide (BLP-NR142).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysates:1. Anti-LRRTM2 (extracellular) Antibody (#ANR-142), (1:200).
2. Anti-LRRTM2 (extracellular) Antibody, preincubated with LRRTM2 (extracellular) Blocking Peptide (BLP-NR142).
Expression of LRRTM2 in mouse parietal cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-LRRTM2 (extracellular) Antibody (#ANR-142), (1:400), followed by goat anti-rabbit-AlexaFluor-488. A. LRRTM2 immunoreactivity (green) appears in cortical neurons in layer 5 (arrows). B. Pre-incubation of the antibody with LRRTM2 (extracellular) Blocking Peptide (BLP-NR142), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CDSQGFHSVPNATDK, corresponding to amino acid residues 47 - 61 of mouse LRRTM2 (Accession Q8BGA3).
Accession (Uniprot) Number Q8BGA3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
