Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPRC5B Blocking Peptide (#BLP-GR072) is the original antigen used for immunization during Anti-GPRC5B Antibody (#AGR-072) generation. The blocking peptide binds and ‘blocks’ Anti-GPRC5B primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-GPRC5B Antibody (#AGR-072), (1:800).
3-4. Anti-GPRC5B Antibody, preincubated with GPRC5B Blocking Peptide (BLP-GR072).
Western blot analysis of human K562 myelogenous leukemia cell line lysate (lanes 1 and 3) and human NCI-H526 lung carcinoma cell line lysates (lanes 2 and 4):1-2. Anti-GPRC5B Antibody (#AGR-072), (1:200).
3-4. Anti-GPRC5B Antibody, preincubated with GPRC5B Blocking Peptide (BLP-GR072).
Expression of GPRC5B in mouse deep cerebellar nuclei.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GPRC5B Antibody (#AGR-072), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GPRC5B immunoreactivity (green) appears in neuron ourlines (arrows). B. Pre-incubation of the antibody with GPRC5B Blocking Peptide (BLP-GR072), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)ENPPNYFDTSQPRMRE, corresponding to amino acid residues 302 - 317 of mouse GPRC5B (Accession Q923Z0).
Accession (Uniprot) Number Q923Z0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
