Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR143 Blocking Peptide (#BLP-GR083) is the original antigen used for immunization during Anti-GPR143 Antibody (#AGR-083) generation. The blocking peptide binds and ‘blocks’ Anti-GPR143 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1-2. Anti-GPR143 Antibody (#AGR-083), (1:500).
3-4. Anti-GPR143 Antibody, preincubated with GPR143 Blocking Peptide (BLP-GR083).
Western blot analysis of rat eye lysate:1. Anti-GPR143 Antibody (#AGR-083), (1:400).
2. Anti-GPR143 Antibody, preincubated with GPR143 Blocking Peptide (BLP-GR083).
Western blot analysis of human ARPE-19 retinal cell epithelium cell line lysate (lanes 1 and 3) and Malme-3M melanoma cell line lysate (lanes 2 and 4):1-2. Anti-GPR143 Antibody (#AGR-083), (1:400).
3-4. Anti-GPR143 Antibody, preincubated with GPR143 Blocking Peptide (BLP-GR083).
Expression of GPR143 in mouse corpus callosum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GPR143 Antibody (#AGR-083), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GPR143 immunoreactivity (green) appears in the corpus callosum (arrows). B. Pre-incubation of the antibody with GPR143 Blocking Peptide (BLP-GR083), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of GPR143 in mouse striatum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GPR143 Antibody (#AGR-083), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GPR143 immunoreactivity (green) appears in the ventral striatum region (arrows). B. Pre-incubation of the antibody with GPR143 Blocking Peptide (BLP-GR083), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)KGRKGVYTENERLMG, corresponding to amino acid residues 225 - 239 of mouse GPR143 (Accession P70259).
Accession (Uniprot) Number P70259
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
