Overview
Type: Synthetic peptide
Form: Lyophilized Powder
TMEM106B (extracellular) Blocking Peptide (#BLP-NR177) is the original antigen used for immunization during Anti-TMEM106B (extracellular) Antibody (#ANR-177) generation. The blocking peptide binds and ‘blocks’ Anti-TMEM106B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-TMEM106B (extracellular) Antibody (#ANR-177), (1:500).
3-4. Anti-TMEM106B (extracellular) Antibody, preincubated with TMEM106B (extracellular) Blocking Peptide (BLP-NR177).
Western blot analysis of human PANC-1 pancreatic duct carcinoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human Jurkat T-cell leukemia cell line lysate (lanes 3 and 6):1-3. Anti-TMEM106B (extracellular) Antibody (#ANR-177), (1:200).
4-6. Anti-TMEM106B (extracellular) Antibody, preincubated with TMEM106B (extracellular) Blocking Peptide (BLP-NR177).
Expression of TMEM106B in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TMEM106B (extracellular) Antibody (#ANR-177), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed TMEM106B immunoreactivity (green) in neurons (arrows). B. Pre-incubation of the antibody with TMEM106B (extracellular) Blocking Peptide (BLP-NR177), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of TMEM106B in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TMEM106B (extracellular) Antibody (#ANR-177), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. TMEM106B immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with TMEM106B (extracellular) Blocking Peptide (BLP-NR177), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)KARLNNITNIGPLDMK, corresponding to amino acid residues 179 - 194 of mouse TMEM106B (Accession Q80X71).
Accession (Uniprot) Number Q80X71
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
