Overview
Type: Synthetic peptide
Form: Lyophilized Powder
CD81 (extracellular) Blocking Peptide (#BLP-NR211) is the original antigen used for immunization during Anti-CD81 (extracellular) Antibody (#ANR-211) generation. The blocking peptide binds and ‘blocks’ Anti-CD81 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and mouse liver lysate (lanes 3 and 6):1-3. Anti-CD81 (extracellular) Antibody (#ANR-211), (1:400).
4-6. Anti-CD81 (extracellular) Antibody, preincubated with CD81 (extracellular) Blocking Peptide (BLP-NR211).
Western blot analysis of human PC3 prostate adenocarcinoma cell line lysates (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):1-2. Anti-CD81 (extracellular) Antibody (#ANR-211), (1:200).
3-4. Anti-CD81 (extracellular) Antibody, preincubated with CD81 (extracellular) Blocking Peptide (BLP-NR211).
Western blot analysis of mouse BV-2 cell line lysate (lane 1) and BV2-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-CD81 (extracellular) Antibody (#ANR-211), (1:200).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lane 1) and U-87 MG-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-CD81 (extracellular) Antibody (#ANR-211), (1:400).
Expression of CD81 in rat medial habenula.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CD81 (extracellular) Antibody (#ANR-211), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CD81 immunoreactivity (green) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with CD81 (extracellular) Blocking Peptide (BLP-NR211), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CHQKIDDLFSGK, corresponding to amino acid residues 190 - 201 of human CD81 (Accession P60033).
Accession (Uniprot) Number P60033
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
