Overview
Type: Synthetic peptide
Form: Lyophilized Powder
CALHM2 (extracellular) Blocking Peptide (#BLP-CC142) is the original antigen used for immunization during Anti-CALHM2 (extracellular) Antibody (#ACC-142) generation. The blocking peptide binds and ‘blocks’ Anti-CALHM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-CALHM2 (extracellular) Antibody (#ACC-142), (1:200).
3-4. Anti-CALHM2 (extracellular) Antibody, preincubated with CALHM2 (extracellular) Blocking Peptide (BLP-CC142).
Western blot analysis of human HMC3 microglia cell line lysate (lanes 1 and 3) and human U-87 MG glioma cell line lysate (lanes 2 and 4):1-2. Anti-CALHM2 (extracellular) Antibody (#ACC-142), (1:200).
3-4. Anti-CALHM2 (extracellular) Antibody, preincubated with CALHM2 (extracellular) Blocking Peptide (BLP-CC142).
Expression of CALHM2 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-CALHM2 (extracellular) Antibody (#ACC-142), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CALHM2 immunoreactivity (green) appears in layers 1-2 of the dorsal horn (vertical arrows) and in some nerve cells (horizontal arrows). B. Pre-incubation of the antibody with CALHM2 (extracellular) Blocking Peptide (BLP-CC142), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)SEFVDPSSLTAGDK, corresponding to amino acid residues 133 - 146 of mouse CALHM2 (Accession Q8VEC4).
Accession (Uniprot) Number Q8VEC4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
