Overview
- Peptide (C)HNETSAHRQKTES, corresponding to amino acid residues 186 - 198 of mouse NPTX2 (Accession O70340). Secreted.
Neuronal Pentraxin 2 (NPTX2) Blocking Peptide (#BLP-NR192)
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain cortex lysate (lanes 2 and 4):1-2. Anti-Neuronal Pentraxin 2 (NPTX2) Antibody (#ANR-192), (1:200).
3-4. Anti-Neuronal Pentraxin 2 (NPTX2) Antibody (#ANR-192), preincubated with Neuronal Pentraxin 2 (NPTX2) Blocking Peptide (#BLP-NR192).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-Neuronal Pentraxin 2 (NPTX2) Antibody (#ANR-192), (1:200).
2. Anti-Neuronal Pentraxin 2 (NPTX2) Antibody (#ANR-192), preincubated with Neuronal Pentraxin 2 (NPTX2) Blocking Peptide (#BLP-NR192).
Expression of NPTX2 in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Neuronal Pentraxin 2 (NPTX2) Antibody (#ANR-192), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NPTX2 immunoreactivity (green) appears in cells of the upper layer (vertical arrows) and in cells of a deeper layer (horizontal arrow). B. Pre-incubation of the antibody with Neuronal Pentraxin 2 (NPTX2) Blocking Peptide (#BLP-NR192), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of NPTX2 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Neuronal Pentraxin 2 (NPTX2) Antibody (#ANR-192), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NPTX2 immunoreactivity (green) appears in cells of the pyramidal layer (P). B. Pre-incubation of the antibody with Neuronal Pentraxin 2 (NPTX2) Blocking Peptide (#BLP-NR192), suppressed staining. Cell nuclei are stained with DAPI (blue).
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Neuronal pentraxin 2 (NP2), also known as NPTX2, NP-II, and neuronal activity-regulated pentraxin (Narp), is a calcium-dependent lectin secreted protein member of the pentraxin family involved in synaptic plasticity and excitatory homeostasis.1,2
NP2 is part of the neuronal pentraxin family that includes two other members: Neuronal Pentraxin 1 (NP1) and Neuronal Pentraxin Receptor (NPTXR). The pentraxin superfamily members are arranged in pentameric structures with a discoid shape. The family members are characterized by a 205 amino acids long conserved sequence located at C-terminal called the pentraxin domain 2,3.
NP2 is widely expressed in the brain, especially in the hippocampus, dentate gyrus, cerebellum, and cerebral cortex regions, as well as in the testis, pancreas, liver, heart, and skeletal muscle.2,3
NP2 interacts with α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, which are crucial for excitatory neurotransmission in the brain. By binding to AMPA receptors, NP2 influences their trafficking and localization at synapses, affecting the strength of excitatory synaptic transmission 2,3.
Given its presence in brain regions associated with learning and memory, alterations in NP2 levels or function have been implicated in cognitive processes. Dysregulation of NP2 has been observed in various neurological disorders, suggesting its potential importance in maintaining cognitive function.4-6
Abnormalities in NP2 expression have been linked to neurodegenerative disorders, including Alzheimer's disease. Changes in NP2 levels may contribute to synaptic dysfunction and cognitive decline observed in these conditions.4-6
Anti-Neuronal Pentraxin 2 (NPTX2) Antibody (#ANR-192) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NPTX2 from rat, mouse and human samples.
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