Overview
Type: Synthetic peptide
Form: Lyophilized Powder
5HT6 Receptor (HTR6) (extracellular) Blocking Peptide (#BLP-SR038) is the original antigen used for immunization during Anti-5HT6 Receptor (HTR6) (extracellular) Antibody (#ASR-038) generation. The blocking peptide binds and ‘blocks’ Anti-5HT6 Receptor (HTR6) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-5HT6 Receptor (HTR6) (extracellular) Antibody (#ASR-038), (1:400).
3-4. Anti-5HT6 Receptor (HTR6) (extracellular) Antibody, preincubated with 5HT6 Receptor (HTR6) (extracellular) Blocking Peptide (BLP-SR038).
Expression of 5HT6 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-5HT6 Receptor (HTR6) (extracellular) Antibody (#ASR-038), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed 5HT6 immunoreactivity (green) in neuronal outlines in the hilus (H, left-pointing arrows) and in the granular layer (G, right pointing arrows). B. Pre-incubation of the antibody with 5HT6 Receptor (HTR6) (extracellular) Blocking Peptide (BLP-SR038), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer, H = hilus.
Expression of 5HT6 in in rat dorsal raphe nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-5HT6 Receptor (HTR6) (extracellular) Antibody (#ASR-038), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. 5HT6 immunoreactivity (green) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with 5HT6 Receptor (HTR6) (extracellular) Blocking Peptide (BLP-SR038), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)HELGKARTPAPGQ(S)R, corresponding to amino acid residues 167 - 181 of rat HTR6 (Accession P31388).
Accession (Uniprot) Number P31388
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
