Overview
Type: Synthetic peptide
Form: Lyophilized Powder
BMPR1A (extracellular) Blocking Peptide (#BLP-BR043) is the original antigen used for immunization during Anti-BMPR1A (extracellular) Antibody (#ABR-043) generation. The blocking peptide binds and ‘blocks’ Anti-BMPR1A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat colon lysate (lanes 1 and 3) and rat small intestine lysate (lanes 2 and 4):1-2. Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:200).
3-4. Anti-BMPR1A (extracellular) Antibody, preincubated with BMPR1A (extracellular) Blocking Peptide (BLP-BR043).
Western blot analysis of mouse brain lysate:1. Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:200).
2. Anti-BMPR1A (extracellular) Antibody, preincubated with BMPR1A (extracellular) Blocking Peptide (BLP-BR043).
Western blot analysis of human HT-29 colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human SW403 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):1-2. Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:200).
3-4. Anti-BMPR1A (extracellular) Antibody, preincubated with BMPR1A (extracellular) Blocking Peptide (BLP-BR043).
Expression of BMPR1A in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. BMPR1A immunoreactivity (green), appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with BMPR1A (extracellular) Blocking Peptide (BLP-BR043), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of BMPR1A in mouse olfactory bulb.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. BMPR1A immunoreactivity (green), appears in cells lined around glomeruli (G, down pointing arrows) and in cells of the mitral layer (up pointing arrows). B. Pre-incubation of the antibody with BMPR1A (extracellular) Blocking Peptide (BLP-BR043), suppressed staining. Cell nuclei are stained with DAPI (blue). G = glomerulus.
Expression of BMPR1A in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. BMPR1A immunoreactivity (green), appears in purkinje cells (arrows). B. Pre-incubation of the antibody with BMPR1A (extracellular) Blocking Peptide (BLP-BR043), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, G = granule layer.
Properties
Sequence
- Peptide (C)DSMLHGTGMKSDLDQK, corresponding to amino acid residues 27 - 42 of mouse BMPR1A (Accession P36895).
Accession (Uniprot) Number P36895
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
