Overview
Type: Synthetic peptide
Form: Lyophilized Powder
CD9 (extracellular) Blocking Peptide (#BLP-NR209) is the original antigen used for immunization during Anti-CD9 (extracellular) Antibody (#ANR-209) generation. The blocking peptide binds and ‘blocks’ Anti-CD9 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
3-4. Anti-CD9 (extracellular) Antibody, preincubated with CD9 (extracellular) Blocking Peptide (BLP-NR209).
Western blot analysis of rat lung lysate:1. Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
2. Anti-CD9 (extracellular) Antibody, preincubated with CD9 (extracellular) Blocking Peptide (BLP-NR209).
Western blot analysis of human THP-1 monocytic leukemia cell line lysates (lanes 1 and 4), human MEG-01 megakaryoblast cell line lysate (lanes 2 and 5) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 6):1-3. Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
4-6. Anti-CD9 (extracellular) Antibody, preincubated with CD9 (extracellular) Blocking Peptide (BLP-NR209).
Western blot analysis of mouse BV-2 cell line lysate (lane 1) and BV-2-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lane 1) and U-87 MG-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-CD9 (extracellular) Antibody (#ANR-209), (1:200).
Expression of CD9 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CD9 (extracellular) Antibody (#ANR-209), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the dentate gyrus region, showed CD9 immunoreactivity (green), in glial profiles in the granule layer (G) and hilus (H). B. Pre-incubation of the antibody with CD9 (extracellular) Blocking Peptide (BLP-NR209), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of CD9 in rat fornix.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CD9 (extracellular) Antibody (#ANR-209), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CD9 immunoreactivity (green), appears in astrocyte profiles (arrows). B. Pre-incubation of the antibody with CD9 (extracellular) Blocking Peptide (BLP-NR209), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of CD9 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CD9 (extracellular) Antibody (#ANR-209), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the CA3 hippocampal region, showed CD9 immunoreactivity (green), in the pyramidal layer (P). B. Pre-incubation of the antibody with CD9 (extracellular) Blocking Peptide (BLP-NR209), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EVIKELQEFYKDTYQK, corresponding to amino acid residues 114 - 129 of mouse CD9 (Accession P40240).
Accession (Uniprot) Number P40240
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
