Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Na+/H+ Exchanger 4 (NHE-4) Blocking Peptide (#BLP-NX004) is the original antigen used for immunization during Anti-Na+/H+ Exchanger 4 (NHE-4) Antibody (#ANX-004) generation. The blocking peptide binds and ‘blocks’ Anti-Na+/H+ Exchanger 4 (NHE-4) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney lysate (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4):1-2. Anti-Na+/H+ Exchanger 4 (NHE-4) Antibody (#ANX-004), (1:400).
3-4. Anti-Na+/H+ Exchanger 4 (NHE-4) Antibody (#ANX-004), preincubated with Na+/H+ Exchanger 4 (NHE-4) Blocking Peptide (BLP-NX004).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-Na+/H+ Exchanger 4 (NHE-4) Antibody (#ANX-004), (1:200).
3-4. Anti-Na+/H+ Exchanger 4 (NHE-4) Antibody (#ANX-004), preincubated with Na+/H+ Exchanger 4 (NHE-4) Blocking Peptide (BLP-NX004).
Expression of NHE-4 in rat brain subfornical organ (SFO).Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Na+/H+ Exchanger 4 (NHE-4) Antibody (#ANX-004), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. NHE-4 immunoreactivity (green) appears in cells of the SFO. B. Pre-incubation of the antibody with Na+/H+ Exchanger 4 (NHE-4) Blocking Peptide (BLP-NX004), suppressed staining. Cell nuclei are stained with DAPI (blue). FX = fornix, SFO= subfornical organ, V = cerebral ventricle
Expression of NHE-4 and Aquaporin-4 in rat brain subfornical organ (SFO).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Na+/H+ Exchanger 4 (NHE-4) Antibody (#ANX-004), (1:200), followed by goat anti-rabbit-AlexaFluor-488 and Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014-GP), (1:200), followed by goat anti-guinea pig-AlexaFluor-594. A. NHE-4 immunoreactivity (green) appears in cells of the SFO. B. AQP4 immunoreactivity (red), appears in cells of the SFO. C. Merge of the two images demonstrates colocalization in several neurons (arrows point at examples). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)ERIQGIKRLSPEDVE, corresponding to amino acid residues 576 - 590 of mouse SLC9A4 (Accession Q8BUE1).
Accession (Uniprot) Number Q8BUE1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
