Overview
Type: Synthetic peptide
Form: Lyophilized Powder
PTGFRN (extracellular) Blocking Peptide (#BLP-NR212) is the original antigen used for immunization during Anti-PTGFRN (extracellular) Antibody (#ANR-212) generation. The blocking peptide binds and ‘blocks’ Anti-PTGFRN (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-PTGFRN (extracellular) Antibody (#ANR-212), (1:400).
3-4. Anti-PTGFRN (extracellular) Antibody, preincubated with PTGFRN (extracellular) Blocking Peptide (BLP-NR212).
Western blot analysis of human pancreatic carcinoma PANC-1 cell line lysate (lanes 1 and 4), human breast adenocarcinoma MCF-7 cell line (lanes 2 and 5) and human U-87 MG glioblastoma cell line (lanes 3 and 6):1-3. Anti-PTGFRN (extracellular) Antibody (#ANR-212), (1:400).
4-6. Anti-PTGFRN (extracellular) Antibody, preincubated with PTGFRN (extracellular) Blocking Peptide (BLP-NR212).
Expression of PTGFRN in mouse hypothalamus:Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-PTGFRN (extracellular) Antibody (#ANR-212), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. PTGFRN immunoreactivity (green) appears in cells of ventromedial hypothalamus (arrows). B. Pre-incubation of the antibody with PTGFRN (extracellular) Blocking Peptide (BLP-NR212), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
Expression of PTGFRN in rat hippocampal dentate gyrus:Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PTGFRN (extracellular) Antibody (#ANR-212), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. PTGFRN immunoreactivity (green) appears in cells of the sub-granular layer (GL). B. Pre-incubation of the antibody with PTGFRN (extracellular) Blocking Peptide (BLP-NR212), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EDEFRYRMYQTQVSD, corresponding to amino acid residues 635 - 649 of mouse PTGFRN (Accession Q9WV91). Extracellular, N-terminus.
Accession (Uniprot) Number Q9WV91
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
