Overview
Type: Synthetic peptide
Form: Lyophilized Powder
IGSF8 (extracellular) Blocking Peptide (#BLP-NR208) is the original antigen used for immunization during Anti-IGSF8 (extracellular) Antibody (#ANR-208) generation. The blocking peptide binds and ‘blocks’ Anti-IGSF8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-IGSF8 (extracellular) Antibody (#ANR-208), (1:200).
3-4. Anti-IGSF8 (extracellular) Antibody, preincubated with IGSF8 (extracellular) Blocking Peptide (BLP-NR208).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human Burkitt's lymphoma (Daudi) cell line lysate (lanes 2 and 6), human normal skin fibroblast Malme-3 cell line lysate (lanes 3 and 7) and Human prostate carcinoma LN-CaP cell line lysate (lanes 4 and 8):1-4. Anti-IGSF8 (extracellular) Antibody (#ANR-208), (1:200).
5-8. Anti-IGSF8 (extracellular) Antibody, preincubated with IGSF8 (extracellular) Blocking Peptide (BLP-NR208).
Properties
Sequence
- Peptide (C)EAGAPYAERLASGELR, corresponding to amino acid residues 227 - 242 of mouse IGSF8 (Accession Q8R366). Extracellular, N-terminus.
Accession (Uniprot) Number Q8R366
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
