Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR173 Blocking Peptide (#BLP-GR093) is the original antigen used for immunization during Anti-GPR173 Antibody (#AGR-093) generation. The blocking peptide binds and ‘blocks’ Anti-GPR173 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of new born rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-GPR173 Antibody (#AGR-093), (1:500).
3-4. Anti-GPR173 Antibody, preincubated with GPR173 Blocking Peptide (#BLP-GR093).
Western blot analysis of human SHSY-5Y neuroblastoma cell lysate (lanes 1 and 4), mouse BV-2 microglia cell lysate (lanes 2 and 5) and human HMC3 microglial cell line lysate (lanes 3 and 6):1-3. Anti-GPR173 Antibody (#AGR-093), (1:500).
4-6. Anti-GPR173 Antibody, preincubated with GPR173 Blocking Peptide (#BLP-GR093).
Expression of GPR173 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR173 Antibody (#AGR-093), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA3 region, showed GPR173 immunoreactivity (green) in cells of the pyramidal layer (arrows). B. Pre-incubation of the antibody with GPR173 Blocking Peptide (BLP-GR093), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.
Expression of GPR173 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR173 Antibody (#AGR-093), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR173 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with GPR173 Blocking Peptide (BLP-GR093), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)GMDEVKGEKQLGR, corresponding to amino acid residues 274 - 286 of mouse GPR173 (Accession Q6PI62).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
