Lysosomes are intracellular acidic organelles containing over 60 different types of hydrolases. They are responsible for the degradation of intracellular components from autophagy and of extracellular particles from endocytosis. Lysosomes contain a large number of membrane proteins, some of them being ion channels which play an important role in regulating membrane potential and lysosomal ionic homeostasis. Thus far the following native ionic conductances have been revealed in single channel recordings and in whole-lysosomal recordings from artificially enlarged lysosomes: K⁺, Na⁺, Cl^–, Ca²⁺ and H⁺.