Technical Service Guide: Immunohistochemistry

Customer Information

First Name

Last Name

Company/Institution

Lab Position

Phone

Product Information

Catalog Number / Product Name

Lot Number

Purchase Date

Sales Order Number

Problem and Previous Experience

What is the specific problem you are experiencing?

Did this same vial of product work in the past? What were the results?

Did other lots of this product work in the past? Which lots? What were the results?

Please attach result images if relevant

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Controls

Has the primary antibody (used in immunohistochemistry) been used successfully in other applications (Western Blot, Immunoprecipitation, Immunocytochemistry)?

What positive and/or negative controls were done and what were the results?

From which species (animal) was the sample?

Samples and Fixation

Did you use Alomone blocking peptide as a negative control? What were the results?

What type of sample was stained (frozen tissue, paraffin-embedded tissue)?

What method of fixation was used (acetone, paraformaldehyde, methanol, other)?

Was Antigen Retrieval performed? If so, by what method (heat treatment, enzyme digestion)?

Blocking

What were the blocking conditions (blocking solution, species of serum, length of incubation)?

Primary Antibody

How was the primary antibody reconstituted and stored?

At what dilution(s) was the primary antibody used and what was the diluent?

What were the incubation conditions (time, temperature) for the primary antibody?

Secondary Antibody

From which company was the secondary antibody obtained and what type of secondary was being used (goat anti-rabbit, goat anti-guinea pig, etc.) and what secondary antibody conjugate was used (biotin, FITC, other)?

At what dilution(s) was the secondary antibody used and what was the diluent?

Detection

What method of detection was used (DAB, Immunofluorescence)?

If a kit was used, was the kit used successfully with other primary antibodies?

In order to process your request as soon as we can, please attach result images below:

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If you tried different experiment conditions, please specify conditions you used and provide the corresponding images.

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Technical Service Guide: Immunohistochemistry