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Antibody Conjugation Services
Our Antibodies - Your Way
Sometimes, what your research needs isn’t in the catalog. Sometimes, you need an antibody conjugated to a specific fluorophore or enzyme to fit into a multiplex assay, or an antibody in a carrier-free solution free from bovine serum albumin (BSA), sodium azide, or glycerol. Sometimes you just need a reagent in bulk or you want to reserve a specific lot for maximum consistency. Now you can easily have all of that with our Antibody Conjugation Services. As a group of scientists who’ve been designing, developing, and validating reagents in-house for decades, we’re confident we can meet your research needs.
Click on the type of service you’re interested in and get your antibodies, your way.
Carrier-Free Antibodies
"Tailored Solutions: Embracing Custom Services to Meet Your Needs"
Carrier-free antibodies enhance the flexibility required for conjugations to fluorophore dyes, biotin, horseradish peroxidase (HRP), metals, etc. There is no need to perform buffer exchange or buy expensive BSA removal kits. Similarly, antibody loss is avoided and time is saved.
Minimum order: 1 x 0.2 ml
Advantages:
- Customization to your exact specifications
- Conjugation ready
- BSA and sodium azide free for consistent conjugation
- Compatibility with a variety of different labels such as metals, fluorophore dyes, biotin, enzymes, etc.
- In vivo applications
- IgGs are affinity-purified in columns with immobilized peptide antigens to ensure the best possible product and an increased conjugation yield
- Antibodies are shipped as a stable lyophilized powder in phosphate-buffered saline (PBS), (pH 7.4)
- Bulk sizes are available
- Progressive pricing for large orders
Applications:
- Compatible with conjugations required for super resolution microscopy (STORM, STED, Confocal)
- Compatible with Cy3 and Cy5 fluorophore conjugations for FRET microscopy
- Compatible with conjugations required for CyTOF assays
- Compatible with biotin conjugations for enzyme-linked immunosorbent assays (ELISAs) that increase the test sensitivity or for signal amplification in western blots and IF applications
- Compatible with enzyme (HRP/AP) conjugations for ELISAs and western blots to be used as detecting antibodies
- Compatible with metal conjugations, such as magnetic beads
Specifications:
- Reconstitution with DDW
- Validation by western blot
- Concentration: 0.8mg/ml
- Volume: 0.2ml
Fluorophore Custom Conjugation and Flow Panel Development
"Brilliantly Customized: Expanding the Spectrum of Fluorophore-Conjugated Antibodies to Meet Your Research Needs"
Primary fluorophore-conjugated antibodies allow direct fluorescent immunodetection methods without the need for a secondary antibody. Direct immunodetection renders your experiment quicker and cheaper while simultaneously reducing non-specific binding of the secondary antibody and producing higher quality data.
Fluorophore reporters: ATTO Fluor-488/550/590/594/633/647N, FITC, R-PE, APC, Pacific Blue, etc.
Minimum order: 4 x 50 µl
Advantages:
- Customization to your exact specifications
- Direct detection shortens protocols, as one-step labelling is utilized
- Direct detection simplifies multicolor staining applications with antibodies from the same species
- Increased flexibility in panel design
- Reduced background signals
- Elimination of secondary antibody cross-reactivity
- Fluorophore-conjugated extracellular primary antibodies make it possible to observe live cells in real time
- Fluorophore-conjugated antibodies are shipped as a stable lyophilized powder in phosphate-buffered saline (PBS), (pH 7.4) 1% BSA, and 0.05% NaN3
- Bulk sizes are available
- Progressive pricing for large orders
Applications:
- Super resolution Microscopy - Immunofluorescence
- Multiplex Immunodetection
- Flow cytometry
- Western blot
- Enzyme-linked immunosorbent assay (ELISA)
Specifications:
- Reconstitution with DDW
- Validation by western blot (pre-conjugation)
- Concentration: 1mg/ml
- Volume: 50µl
A rabbit IgG isotype control can be fluorescently labeled for your specific application
Live intact human megakaryoblastic MEG-01 cells were stained simultaneously with 5 μg of the following antibodies: Anti-P2Y1 Receptor (extracellular)-Pacific Blue Antibody, Anti-TRPC6 (extracellular)-FITC Antibody, Anti-P2Y12 Receptor (extracellular)-PE Antibody, and Anti-PAR4 (F2RL3) (extracellular)-APC Antibody.
| Laser | Filter | Antibody Name | Label |
|---|---|---|---|
| Violet (405 nm) | 450/50 | Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) | Pacific Blue |
| Blue (488 nm) | 530/30 | Anti-TRPC6 (extracellular)-FITC Antibody (#ACC-120-F) | FITC |
| Yellow-Green (561 nm) | 586/15 | Anti-P2Y12 Receptor (extracellular)-PE Antibody (#APR-020-PE) | PE |
| Red (637 nm) | 670/30 | Anti-PAR4 (F2RL3) (extracellular)-APC Antibody (#APR-034-APC) | APC |
Custom Biotin Conjugation
"Signal Amplifiers: Enhancing Antibodies Through Biotin Conjugation"
Primary biotin-conjugated antibodies produce an amplified immunodetection by binding streptavidin molecules and can be used for many applications. Amplified signals allow detection of proteins expressed at low levels, increasing the sensitivity of immunodetection tests.
Minimum order: 4 x 50 µl
Advantages:
- Signal amplification
- Detection of low-abundance proteins
- High sensitivity
- Customization to your exact specifications
- Biotin-conjugated antibodies are shipped as a stable lyophilized powder in phosphate-buffered saline (PBS) (pH 7.4), 1% BSA, and 0.05% NaN3
- Bulk sizes are available
- Progressive pricing for large orders
Applications:
- Immunofluorescence
- Western blot
- Enzyme-linked immunosorbent assay (ELISA)
Specifications:
- Reconstitution with DDW
- Validation by western blot
- Concentration: 1mg/ml
- Volume: 50µl
"Enhancing Detection With Enzyme Conjugation"
Primary antibodies conjugated to enzymes such as HRP or alkaline phosphatase serve as immunodetectors for enzyme-linked immunosorbent assays (ELISAs), western blots, and immunohistochemistry.
Minimum order: 4 x 50 µl
Advantages:
- Customization to your exact specifications
- Direct detection shortens protocols as one-step labelling is utilized
- No need for secondary antibody
- Enzyme-conjugated antibodies are shipped as a stable lyophilized powder in phosphate-buffered saline (PBS) (pH 7.4)
- Bulk sizes are available
- Progressive pricing for large orders
Applications:
- Immunohistochemistry
- Western blot
- ELISA
Specifications:
- Reconstitution with DDW
- Validation by western blot
- Concentration: 1mg/ml
- Volume: 50µl