Multiplex immunofluorescence (IF) or immunostaining, sometimes also referred to as immuno co-localization involves the sequential application of conjugated antibodies. The aim of the technique is to help determine the co-occurrence of two or more proteins, typically in the same sub-cellular structure or tissue.
Alomone labs offers a number of directly conjugated primary antibodies to help streamline your multiplex immunostaining experiment – excluding the need for a secondary antibody detection. Our primary conjugates of choice are a new generation of fluorophores – ATTO dyes*. We optimize and test them in house for multiplex immunostaining.
They offer several advantages over many commonly used conjugate dyes, like Fluorescein isothiocyanate (FITC) and Alexa Fluor®️.
- Stronger absorption (high extinction coefficient)
- Higher fluorescence quantum yield
- Higher photo-stability
For more information on ATTO dye applications:
Conjugated antibody guide for multiplex immunostaining
We have ~100 ATTO-conjugated primary antibodies, which we have used in combination with our Guinea Pig-raised polyclonal antibodies for multiplex immunostaining (immuno co-localization). The use of guinea pig-raised antibodies helps minimize cross-reactivity between antibodies where the host species are often mouse, rabbit or goat.
Explore our complete product portfolio for Multiplex staining
View our relevant protocols
* Used under license from from ATTO-TEC GmBH https://www.atto-tec.com