Overview
Alternative Name β-PMTX
Lyophilized Powder yes
Origin Synthetic peptide
MW: 1559 Da
Purity: >98% (HPLC)
Effective concentration 10-100 µM.
Sequence RIKIGLFDQLSRL.
Modifications Lys13 - C-terminal amidation.
Structure
Molecular formula C71H124N22O17.
CAS No.: 216064-36-7
Activity β-Pompilidotoxin slows NaV channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse1-3. β-Pompilidotoxin is five times more potent than α-Pompilidotoxin1,2.
References-Activity
- Sahara, Y. et al. (2000) Eur. J. Neurosci. 12, 1961.
- Konno, K. et al. (2000) Neurosci. Lett. 285, 29.
- Konno, K. et al. (1998) Biochem. Biophys. Res. Commun. 250, 612.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Centrifuge all products BEFORE adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial and tap on it to dissolve lyophilized peptide. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing (3-5 seconds of light vortexing is acceptable). Confirm visually that the solution is clear. Spin down and make sure no visible pellet accumulates in the vial (to ensure complete dissolution). Divide the solution into single-use aliquots and store at -20°C. Before use, thaw the relevant vial(s) intended for use and dilute in the desired working buffer. Avoid multiple freeze-thaw cycles to maintain biological activity. The preparation of fresh solutions in working buffers before use is recommended.
Soluble in pure water up to 10 mM. For long-term storage in solution, it is recommended to prepare a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration.
Soluble in pure water up to 10 mM. For long-term storage in solution, it is recommended to prepare a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration.
Storage of solutions Up to one week at 4°C or three months at -20°C.
Our bioassay
β-Pompilidotoxin enhances native Nav currents in ND7-23 cells.The effect on inward currents of a bath application of 100 µM β-Pompilidotoxin (#P-180), (cyan). Nav currents were elicited every 15 sec. from a holding potential of -100 mV, by 20 ms test pulses to -10 mV.
Alomone Labs β-Pompilidotoxin enhances NaV1.6 currents heterologously expressed in Xenopus oocytes.A. Time course of β-Pompilidotoxin (#P-180) action on NaV1.6 currents. Current area was plotted as a function of time. Membrane potential was held at -80 mV and oocytes were stimulated by a 100 ms voltage step to 0 mV, applied every 10 sec using two electrode voltage clamp. 5 μM (green) or 20 μM (magenta) β-Pompilidotoxin were perfused as indicated by the horizontal bars.
B. Superimposed traces of NaV1.6 currents in the absence (control) and presence of 5 μM (green) or 20 μM (magenta) β-Pompilidotoxin.
References - Scientific background
- Sahara, Y. et al. (2000) Eur. J. Neurosci. 12, 1961.
- Konno, K. et al. (2000) Neurosci. Lett. 285, 29.
- Konno, K. et al. (1998) Biochem. Biophys.Res. Commun. 250, 612.
Scientific background
β-PMTX was originally isolated from Anoplius samariensis and Batozonellus maclifrons wasps. This is a synthetic version of the peptide.
β-PMTX is a homologous peptide to α-PMTX (position 12 is Arginine instead of Lysine). β-PMTX induces repetitive action potentials and facilitates both excitatory and inhibitory synaptic transmission.
Recently it was found that this toxin slows the Na+-channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse of the lobster walking leg and in the rat trigeminal ganglion neurons.1-3 β-PMTX is five times more potent than α-PMTX.1,2
Target TTX-sensitive Na+ channels
Peptide Content: 100%
Lyophilized Powder
β-Pompilidotoxin (#P-180) is a highly pure, synthetic, and biologically active peptide toxin.
For research purposes only, not for human use
Last Update: 30/05/2024
Applications
Citations
Citations
Powered by BiozProduct citations
- Gilchrist, J. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, E5016.
