Overview
Alternative Name Mu-Conotoxin CnIIIC
Lyophilized Powder yes
Origin Synthetic peptide
MW: 2376 Da
Purity: >99% (HPLC)
Effective concentration 0.5-1 µM.
Sequence ZGCCNGPKGCSSKWCRDHARCC.
Modifications Disulfide bonds between Cys3-Cys15, Cys4-Cys21 and Cys10-Cys22. Cys22 – C-terminal amidation. Z-Pyrrolidone carboxylic acid.
Structure
Molecular formula C92H139N35O28S6
Activity µ-Conotoxin CnIIIC blocks NaV channels and α3/β2 nAChRs.
Accession number I1SB07.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs µ-Conotoxin CnIIIC inhibits NaV1.2 currents heterologously expressed in Xenopus oocytes.A. Time course of µ-Conotoxin CnIIIC (#STC-640) action on peak current NaV1.2 amplitude. Peak current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage step to -10 mV. 0.5 µM µ-Conotoxin CnIIIC was perfused as indicated by the bar for 5 min. B. Superimposed examples of NaV1.2 channel peak current in the absence (control) and presence (green) of 0.5 µM µ-Conotoxin-CnIIIC (taken from the experiment in A).
Scientific background µ-Conotoxin CnIIIC, a 22-residue conopeptide from Conus consors belonging to µ-conopeptide family, is a very potent, selective and durable antagonist of NaV channels. This toxin blocks both voltage-gated Na+ channels and neuronal nicotinic acetylcholine receptor (nAChR). µ-Conotoxin CnIIIC inhibits the rat skeletal muscle NaV1.4 with IC50 of 1.3 nM and the rat brain NaV1.2 expressed in HEK-293 cells. A low inhibition is also observed for mouse neuronal NaV1.6 and mouse NaV1.7. µ-Conotoxin CnIIIC modestly blocks nAChR α3/β2 subtype with IC50 values of 450 nM (partially reversible) and, to a lesser extent, α7 and α4/β2 subtypes (reversible) expressed in Xenopus oocytes. in vitro, it decreases twitch tension in mouse hemidiaphragms (IC50 = 150 nM) and displays a high blocking effect in mouse extensor digitorum longus muscles (IC50 = 46 nM).
Target NaV channels, α3/β2 nAChR
Peptide Content: 100%
Lyophilized Powder
For research purposes only, not for human use
Last Update: 06/10/2024
Specifications
Citations
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