Overview
Alternative Name Myotoxin II, Geographutoxin II, GTx-II
Lyophilized Powder yes
Origin Synthetic peptide
MW: 2640 Da
Purity: >99% (HPLC)
Effective concentration 100-600 nM.
Sequence RDCCTXXRKCKDRRCKXMKCCA.
Modifications Disulfide bonds between Cys3-Cys15, Cys4-Cys20, and Cys10-Cys21. X = 4-Hydroxyproline. Ala22 - C-terminal amidation.
Structure
Molecular formula C101H175N39O30S7.
CAS No.: 140678-12-2
Activity μ-Conotoxin GIIIB binds and blocks site 1 of voltage-gated Na+ channels1.
References-Activity
- Cruz, L.J. et al. (1985) J. Biol. Chem. 260, 9280.
Accession number P01524
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to one week at 4°C or three months at -20°C.
Our bioassay
Alomone Labs µ-Conotoxin GIIIB inhibits NaV1.4 channel currents heterologously expressed in Xenopus oocytes.A. Representative time course of µ-Conotoxin GIIIB (#C-270) inhibition of NaV1.4 channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage step to 0 mV every 10 sec, and significantly inhibited by application of 100 nM µ-Conotoxin GIIIB (green).
B. Superimposed traces of NaV1.4 channel currents in the absence (control) and presence (green) of 100 nM µ-Conotoxin GIIIB (taken from the recording in A).
Scientific background µ-Conotoxin GIIIB was originally isolated from the marine snail Conus geographus. The peptide toxin selectively blocks NaV1.4 channels, which are expressed predominantly in skeletal muscle.1,2 It is a potent blocker and 600 nM completely blocked NaV1.4-related currents in rat skeletal muscle.3
Target NaV1.4 Na+ channels
Peptide Content: 100%
Lyophilized Powder
Image & Title
EDL muscle fibers from SBMA mice show reduced sensitivity to µ-Conotoxin GIIIB.Traces of action potentials before and after incubation in 2.5 µM µ-Conotoxin GIIIB (#C-270) for 30 minutes. Potentials were blocked in WT fibers (left) but not in diseased fibers (right).Adapted from Xu, Y. et al. (2016) J. Neurosci. 36, 5094. with permission of The Society for Neuroscience.
For research purposes only, not for human use
Last Update: 23/09/2024
Specifications
Citations
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