Overview
Alternative Name μ-Conotoxin P3.7
Lyophilized Powder yes
Origin Synthetic peptide
MW: 2604 Da
Purity: >98% (HPLC)
Effective concentration 10 nM - 1 μM.
Sequence QRLCCGFPKSCRSRQCKPHRCC.
Modifications Disulfide bonds between Cys4-Cys16, Cys5-Cys21, and Cys11-Cys22. Cys22 - C-terminal amidation, Hydroxyprolines at Pro8 and Pro18 and Pyrrolidone carboxylic acid at Q1.
Structure
Molecular formula C103H167N41O27S6
Activity μ-Conotoxin PIIIA reversibly blocks mammalian skeletal muscle voltage-gated Na+ channels (NaV1.4, IC50 of 44 nM), Type II (NaV1.2) and PN1 (NaV1.7) neuronal voltage-gated Na+ channels (IC50 of 640 nM) by binding to site I domain of the channel. In addition, it blocks irreversibly amphibian muscle Na+ channels1.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs μ-Conotoxin PIIIA inhibits NaV1.4 channel currents expressed in Xenopus oocytes.NaV1.4 currents were elicited by application of voltage ramp of 60 ms from holding potential of -100 mV to +40 mV. A. Time course of peak current amplitude before (black) and during (green) application of 20 nM μ-Conotoxin PIIIA (#STC-400) applied for 180 s. B. Superimposed example current traces before (black) and during (green) application of the toxin, taken from the experiment in A.
References - Scientific background
- Shon, K.J. et al. (1998) J. Neuroscience 18, 4473.
- Nielsen, K.J. et al. (2002) J. Biol. Chem. 277, 27247.
- Safo, P. et al. (2000) J. Neurosci. 20, 76.
Scientific background µ-Conotoxin PIIIA is a 22 amino acid peptidyl toxin isolated from the Conus purpurascens (Purple cone) venom and belongs to the conotoxin M superfamily1. It reversibly blocks mammalian skeletal muscle voltage-gated Na+ channels (NaV1.4, IC50 of 44 nM), Type II (NaV1.2) and PN1 (NaV1.7) neuronal voltage-gated Na+ channels (IC50 of 640 nM) by binding to site I domain of the channel1-3. In addition, it irreversibly blocks amphibian muscle Na+ channels1.
Target Various NaV channels
Peptide Content: 100%
Lyophilized Powder
µ-Conotoxin PIIIA (#STC-400) is a highly pure, synthetic, and biologically active peptide toxin.
For research purposes only, not for human use
Last Update: 06/10/2024
Applications
Citations
Citations
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- Feng, B. et al. (2015) J. Neurophysiol. 113, 2618.
- Kirchhof, P. et al. (2015) Circ. Heart Fail. 8, 79.
