µ-Conotoxin SIIIA

Applications
Specifications
Scientific Background

Overview

Cat #: RTM-100

Purity: >98% (HPLC)

MW: 2207 Da

Form: Lyophilized

µ-Conotoxin SIIIA (#STC-450) is a highly pure, synthetic, and biologically active peptide toxin.

Certificate of Analysis SDS

Structure

For research purposes only, not for human use

Applications

Citations (60)

Citations

Specifications

Properties

Technical Specifications

Origin Synthetic peptide

MW 2207 Da

Sequence ZNCCNGGCSSKWCRDHARCC.

Modifications Disulfide bonds between Cys³-Cys¹³, Cys⁴-Cys¹⁹, Cys⁸-Cys²⁰_. Z¹ - Pyrrolidone carboxylic acid, Cys²⁰ - Cysteine amide.

Peptide Content 100%

Purity >98% (HPLC)

Molecular formula C₈₃H₁₂₃N₃₃O₂₇S₆.

Accession number Q86DU6

Biological Activity

Target Na_V1.2, Na_V1.3, Na_V1.4 Na⁺ channels

Effective concentration 0.5-3 µM.

Activity µ-Conotoxin SIIIA blocks TTX-sensitive voltage-gated Na⁺ channels in small-diameter DRG neurons^1,2. It also irreversibly blocks Na_V1.2, Na_V1.3 and Na_V1.4 channels expressed in Xenopus oocytes in a dose- and time-dependent manner³. No inhibition is detected on Na_V1.5, Na_V1.7 or Na_V1.8 channels^4,5.

References

Bulaj, G. et al. (2005) Biochemistry 44, 7259.
Wang, C.Z. et al. (2006) Toxicon 47, 122.
Schroeder, C.I. et al. (2008) J. Biol. Chem. 283, 21621.
Yao, S. et al. (2008) Biochemistry 47, 10940.
Leipold, E. et al. (2011) Neuropharmacology 61, 105.

Solubility and Storage

Shipping and storage The product is shipped as a lyophilized powder at room temperature. Upon receipt, it should be stored at -20°C. Protect from moisture.

Solubility Soluble in water. For long-term storage in solution, we recommend to prepare a stock solution by dissolving the product in ddH2O at a concentration X100-1000 of final working solution. Divide the solution into small aliquots and store at -20°C. Upon use, thaw the relevant vial intended for use and dilute in your desired working buffer. The preparation of fresh solutions in working buffers before use is recommended. Repeat freeze-thawing might result in loss of activity. Centrifuge all product preparations before use (10000 x g, 5 min).

Storage of solutions The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the toxin in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Scientific Background

References

Scientific background µ-Conotoxin SIIIA is a synthetic peptide purified to homogeneity, originally isolated from the Conus striatus venom¹. µ-Conotoxin SIIIA reversibly blocks TTX-sensitive voltage-gated Na⁺ channels in small-diameter DRG neurons.^1,2 It also irreversibly blocks Na_V1.2, Na_V1.3 and Na_V1.4 channels expressed in Xenopus oocytes in a dose- and time-dependent manner³. No inhibition is detected on Na_V1.5, Na_V1.7 or Na_V1.8 channels^4,5.

References