Overview
Alternative Name α-Conotoxin G1
Lyophilized Powder yes
Origin Synthetic peptide
MW: 1438 Da
Purity: >98% (HPLC)
Effective concentration 10 nM - 1 μM.
Sequence ECCNPACGRHYSC-NH2
Modifications Disulfide bonds between: Cys2-Cys7, Cys3-Cys13
Cys13 - Cysteine amide
Cys13 - Cysteine amide
Structure
Molecular formula C55H80N20O18S4
CAS No.: 76862-65-2
Activity α-Conotoxin GI inhibits α/δ site on mouse muscle-derived BC3H-1 nicotinic acetylcholine receptors (nAChR) expressed on postsynaptic membranes. Other site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI1,2.
References-Activity
- Groebe, D.R. et al. (1995) Mol. Pharmacol. 48, 105.
- Groebe, D.R. et al. (1997) Biochemistry 36, 6469.
Accession number
Shipping and storage The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Solubility Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed.
The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer.
Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer.
Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Storage of solutions The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Our bioassay
Alomone Labs α-Conotoxin GI inhibits muscle fetal α1/β1/γ/δ nAChR heterologously expressed in Xenopus oocytes.A. Time course of α-Conotoxin GI (#STC-500) action on muscle α1/β1/γ/δ nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at –80 mV and oocytes were stimulated by exposure to 100 µM acetylcholine every 50 seconds. 100 nM (green) α-Conotoxin GI significantly inhibits the currents. B. Superimposed traces of α1/β1/γ/δ nAChR currents evoked by ACh (arrow) after application of control (black) and 100 nM (green) α-Conotoxin GI (taken from the recording in A).
References - Scientific background
- Gray, W.R. et al. (1981) The J. Biol. Chem. 256, 4734.
- Nishiuchi, Y. and Sakakibara, S. (1982) FEBS LETT. 148, 260.
- Guddat, L.W. et al. (1996) Biochemistry 35, 11329.
- Groebe, D.R. et al. (1995) Mol. Pharmacol. 48, 105.
- Groebe, D.R. et al. (1997) Biochemistry 36, 6469.
Scientific background
α-Conotoxin GI is a 13 amino acid peptidyl toxin isolated from the Conus geographus (Geography cone) venom1,2,3. It belongs to the Conotoxin A superfamily and reversibly blocks α/δ nicotinic ACh channel receptors (nAChR).
α-Conotoxin GI reversibly inhibits the high affinity α/δ site on mouse muscle-derived BC3H-1 receptor, a nAChR expressed on postsynaptic membranes4,5. Other low site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI5.
Target α1/β1/δ/γ nAChR
Peptide Content: 100%
Lyophilized Powder
α-Conotoxin GI (#STC-500) is a highly pure, synthetic, and biologically active peptide toxin.
For research purposes only, not for human use
Last Update: 23/03/2025
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