Overview
Alternative Name α-CTx ImI
Lyophilized Powder yes
Origin Synthetic peptide
MW: 1351 Da
Purity: >99% (HPLC)
Effective concentration 0.1-10 μM
Sequence GCCSDPRCAWRC
Modifications Disulfide bonds between Cys2-Cys8 and Cys3-Cys12. Cys12 - C-terminal amidation.
Structure
Molecular formula C52H78N20O15S4.
CAS No.: 156467-85-5
Activity α-Conotoxin ImI potently and specifically blocks mammalian neuronal nAChR (α3/β2 > α7 > α3/β4)1.
References-Activity
- McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
Accession number
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs α-Conotoxin ImI inhibits α3/β2 nAChRs expressed in Xenopus oocytes.A. Representative time course of α3/β2 current inhibition by 100 nM (green) and 1 µM (magenta) α-Conotoxin ImI (#C-290). Currents were elicited every 50 sec by transient applications of 2 µM ACh, while membrane potential was held at -60 mV, and inhibited by 100nM and 1 µM α-Conotoxin ImI.
B. Superimposed traces of α3/β2 currents evoked by ACh (arrow) after application of control (black), 100nM (green) and 1 µM (magenta) α-Conotoxin ImI (Taken from the recording in A).
References - Scientific background
- McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
- Johnson, D.S. et al. (1995) Mol. Pharmacol. 48, 194.
Scientific background α-Conotoxin ImI is a 12 amino acid peptidyl toxin isolated from Conus Imperialis venom1, which blocks mammalian neuronal nAChRs located on postsynaptic membranes. This toxin inhibits mammalian neuronal nAChRs (α3/β2 > α7 > α3/β4) in a voltage-independent manner and has no effect on nAChRs composed of α2/β2, α3/β2, α4/β2, α2/β4, α3/β4, or α4/β4 subunits1,2.
Target α3/β2 nAChR
Peptide Content: 100%
Lyophilized Powder
α-Conotoxin ImI (#C-290) is a highly pure, synthetic, and biologically active peptide toxin.
For research purposes only, not for human use
Last Update: 07/05/2024
Applications
Citations
Citations
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- Kiss, T. et al. (2014) PLoS ONE 9, e109538.
