Overview
Type: Synthetic peptide
Form: Lyophilized powder
α1-Syntrophin/SNTA1 Blocking Peptide (#BLP-PZ021) is the original antigen used for immunization during Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021) generation. The blocking peptide binds and ‘blocks’ Anti-α1-Syntrophin/SNTA1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse heart lysate (lanes 1 and 3) and rat skeletal muscle membranes (lanes 2 and 4):1,2. Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:400).
3,4. Anti-α1-Syntrophin (SNTA1) Antibody, preincubated with α1-Syntrophin/SNTA1 Blocking Peptide (#BLP-PZ021).
Western blot analysis of mouse brain lysate:1. Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:200).
2. Anti-α1-Syntrophin (SNTA1) Antibody, preincubated with α1-Syntrophin/SNTA1 Blocking Peptide (#BLP-PZ021).
Expression of Syntrophin alpha 1 in mouse neocortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:400), followed by anti-rabbit-AlexaFluor-488 antibody (green). Syntrophin alpha 1 staining (green) appears in blood vessels (horizontal arrows) and in the pia mater (vertical arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RQPSSPGPQPRNLSE, corresponding to amino acid residues 191-205 of mouse α1-syntrophin (Accession Q61234).
Accession (Uniprot) Number Q61234
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
