Overview
Type: Synthetic peptide
Form: Lyophilized powder
α1D-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR019) is the original antigen used for immunization during Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019) generation. The blocking peptide binds and ‘blocks’ Anti-α1D-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain stem (lanes 1 and 3), mouse brain (lanes 2 and 4), SH-SY5Y (lanes 5 and 7) and Jurkat (lanes 6 and 8) lysates:1,2,5,6. Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:200).
3,4,7,8. Anti-α1D-Adrenergic Receptor (extracellular) Antibody, preincubated with α1D-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR019).
Expression of α1D-Adrenoceptor in rat heartImmunohistochemical staining of rat heart paraffin embedded sections using Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:100). α1D-Adrenoceptor is expressed in cardiomyocytes of the myocardium and in the smooth muscle of the blood vessels (arrows). Hematoxilin is used as the counterstain.
Expression of α1D-Adrenoceptor in rat neocortexImmunohistochemical staining of α1D-adrenoceptor in rat neocortex using Anti-α1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019). A. Most intense staining of α1D-adrenoceptor (green) appears in apical dendrites (thin horizontal arrows) but also in the soma (thick horizontal arrow). Few cortical interneurons express α1D-adrenoceptor. B. The same section was also stained for parvalbumin and one cell (marked with a vertical arrow) also expresses α1D-adrenoceptor.
Properties
Sequence
- (C)EPVPPDERF*SGITEE, corresponding to amino acid residues 231-245 of rat α1D-adrenoceptor with replacement of cysteine 240 (C240) with serine (*S) (Accession P23944).
Accession (Uniprot) Number P23944
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
