Overview
Type: Synthetic peptide
Form: Lyophilized powder
α2A-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR020) is the original antigen used for immunization during Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020) generation. The blocking peptide binds and ‘blocks’ Anti-α2A-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1, 2. Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020), (1:200).
3, 4. Anti-α2A-Adrenergic Receptor (extracellular) Antibody, preincubated with α2A-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR020).
Expression of α2A-Adrenoreceptor in rat cerebellumImmunohistochemical staining of rat cerebellum free floating frozen section using Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020). A. α2A-Adrenoreceptor staining (green) appears in processes of Bergmann glia (white arrows) in the molecular layer (ML). B. Staining of the same section with mouse anti glial fibrillary acidic protein (GFAP) (red), demonstrates expression in both Bergmann glia and in granule layer (GL) astrocytes. C. Merged image of panel A and B demonstrating that α2A-adrenoreceptor expression is restricted to Bergmann glia. DAPI is used as the counterstain (blue).
Expression of α2A-Adrenoreceptor in rat hypothalamusImmunohistochemical staining of rat ventromedial hypothalamus free floating frozen section using Anti-α2A-Adrenergic Receptor (extracellular) Antibody (#AAR-020), (1:250). A. α2A-Adrenoreceptor staining (green) appears in astrocytic fibers (arrows) originating from the wall of the 3rd ventricle (3rd V). B. DAPI is used as the counterstain (blue). C. Merged image of panels A and B.
Properties
Sequence
- (C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of rat α2A-adrenoceptor (Accession P22909).
Accession (Uniprot) Number P22909
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
