Overview
Type: Synthetic peptide
Form: Lyophilized powder
α2B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR021) is the original antigen used for immunization during Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021) generation. The blocking peptide binds and ‘blocks’ Anti-α2B-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 5), lung (lanes 2 and 6), liver (lanes 3 and 7) lysates and rat skeletal muscle membranes (lanes 4 and 8):1-4. Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), (1:200).
5-8. Anti-α2B-Adrenergic Receptor (extracellular) Antibody, preincubated with α2B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR021).
Expression of α2B-Adrenoreceptor in rat lungImmunohistochemical staining of rat lung paraffin embedded sections using Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), (1:100). α2B-Adrenoreceptor is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.
Expression of α2B-Adrenergic Receptor in mouse cerebellar nuclei.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. α2B-Adrenergic Receptor immunoreactivity (green) appears in neuronal profiles. B. Pre-incubation of the antibody with α2B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR021), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of α2B-Adrenergic Receptor in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. α2B-Adrenergic Receptor immunoreactivity (green) appears in Purkinje cells (arrows) and in the molecular layer (M). B. Pre-incubation of the antibody with α2B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR021), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, G = granule layer.
Properties
Sequence
- (C)RPEPRGLPQ*SELNQE, corresponding to amino acid residues 160-174 of rat α2B-adrenoceptor with replacement of cysteine 169 (C169) with serine (*S) (Accession P19328).
Accession (Uniprot) Number P19328
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
