Overview
Type: Synthetic peptide
Form: Lyophilized powder
α2C-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR022) is the original antigen used for immunization during Anti-α2C-Adrenergic Receptor (extracellular) Antibody (#AAR-022) generation. The blocking peptide binds and ‘blocks’ Anti-α2C-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 4), kidney (lanes 2 and 5) and brain (lanes 3 and 6) membranes:1-3. Anti-α2C-Adrenergic Receptor (extracellular) Antibody (#AAR-022), (1:200).
4-6. Anti-α2C-Adrenergic Receptor (extracellular) Antibody, preincubated with α2C-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR022).
Expression of α2C-Adrenoreceptor in rat kidneyImmunohistochemical staining of rat kidney paraffin embedded sections using Anti-α2C-Adrenergic Receptor (extracellular) Antibody (#AAR-022), (1:100). α2C-Adrenoreceptor is expressed in both proximal (PT) and distal (DT) renal tubules and in the collecting ducts (CD). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)RRPDGAAYPQ*SGLNDE, corresponding to amino acid residues 192-207 of rat α2C-adrenoceptor with replacement of cysteine 202 (C202) with serine (*S) (Accession P22086).
Accession (Uniprot) Number P22086
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
