Overview
Type: Synthetic peptide
Form: Lyophilized powder
β 2 Na+/K+ ATPase (extracellular) Blocking Peptide (#BLP-NP012) is the original antigen used for immunization during Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012) generation. The blocking peptide binds and ‘blocks’ Anti-β 2 Na+/K+ ATPase (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012), (1:400).
2. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody, preincubated with β 2 Na+/K+ ATPase (extracellular) Blocking Peptide (#BLP-NP012).
Western blot analysis of mouse brain lysate:1. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012), (1:200).
2. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody, preincubated with β 2 Na+/K+ ATPase (extracellular) Blocking Peptide (#BLP-NP012).
Expression of Na+/K+ ATPase β2 (ATP1B2) in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012), (1:300), followed by goat anti-rabbit-AlexaFluor-488. ATP1B2 staining (green) in the hippocampal CA1 region, is detected in glial cell profiles (horizontal arrows) in the stratum radiatum (SR) and pyramidal (Pyr) layer. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DTESWDQHVQKLNK, corresponding to amino acid residues 99-112 of rat ATP1B2 (Accession P13638).
Accession (Uniprot) Number P13638
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
