Overview
Type: Synthetic peptide
Form: Lyophilized powder
β3-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR017) is the original antigen used for immunization during Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017) generation. The blocking peptide binds and ‘blocks’ Anti-β3-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,3. Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017), (1:200).
2,4. Anti-β3-Adrenergic Receptor (extracellular) Antibody, preincubated with β3-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR017).
Expression of β3-Adrenoceptor in rat hippocampusImmunohistochemical staining of rat hippocampal dentate gyrus using Anti-β3-Adrenergic Receptor (extracellular) Antibody (#AAR-017), (1:100), (green). β3-Adrenoceptor is strongly expressed in the hilus (H) and in the outer molecular layer (arrows). The distribution overlaps the entire layers, but is not restricted to nerve cells (stained red with mouse anti-Parvalbumin). DAPI is used as the counterstain (blue).
Properties
Sequence
- DAEAQESH*SNPRC, corresponding to amino acid residues 180-192 of mouse ADRB3 with replacement of cysteine 186 (C186) with serine (*S) (Accession P25962).
Accession (Uniprot) Number P25962
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
