Overview
Alternative Name κ-M-RIIIJ
Lyophilized Powder yes
Origin Synthetic peptide
MW: 2807.9 Da
Purity: >98% (HPLC)
Effective concentration 30 nM.
Sequence LXXCCTXXKKHCXAXACKYKXCCKS.
Modifications Disulfide bonds between Cys4-Cys17, Cys5-Cys22 and Cys12-Cys23. X = Hydroxyproline.
Structure
Molecular formula C117H184N32O36S6.
Activity κ-Conotoxin RIIIJ is a blocker of KV1.2 channels1.
References-Activity
- Chen, P. et al. (2010) J. Biol. Chem. 285, 14882.
Accession number P0CG45.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs κ-Conotoxin RIIIJ inhibits KV1.2 currents in Xenopus oocytes.A. Time course of κ-Conotoxin-RIIIJ (#STC-660) action on Kv1.2 currents. Current amplitudes were plotted as a function of time. Membrane potential was held at –80 mV and oocytes were stimulated by a 100 ms voltage ramp to 0 mV. 50 nM κ-Conotoxin-RIIIJ were perfused as indicated by the bar (green) at -15 mV for 160 sec. B. Superimposed examples of KV1.2 channel current in the absence (control) and presence of 50 nM κ-Conotoxin-RIIIJ (taken from the experiment in A).
References - Scientific background
- Hille, B. (1992) Ionic Channels of Excitable Membranes, 2nd Ed. Sunderland, MA: Sinauer Associates.
- Gulbis J. M. et al. (2002) Novartis Found. Symp. 245, 127
- Chen, P. et al. (2010) J. Biol. Chem. 285, 14882.
Scientific background
κ-conotoxin RIIIJ (κ-M-RIIIJ), is a peptide toxin belonging to the conotoxin M superfamily, and was originally isolated from Conus radiatus (Rayed cone) venom. This conopeptide inhibits the homotetrameric human KV1.2 channels (IC50 = 33 nM)3. κ-M-RIIIJ was found to exert cardioprotective effects, presumably due to the high affinity of this peptide for mammalian KV1.2 and KV1.2- containing heteromultimers3.
Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1,2.
Target KV1.2 K+ channels
Peptide Content: 100%
Lyophilized Powder
κ-Conotoxin RIIIJ (#STC-660) is a highly pure, synthetic, and biologically active peptide toxin.
For research purposes only, not for human use
Last Update: 20/10/2024
Applications
Citations
Citations
Powered by BiozProduct citations
- Smith, K.E. et al. (2015) J. Neurosci. 35, 11221.
