Overview
Type: Synthetic peptide
Form: Lyophilized powder
5HT2B Receptor/HTR2B (extracellular) Blocking Peptide (#BLP-SR035) is the original antigen used for immunization during Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035) generation. The blocking peptide binds and ‘blocks’ Anti-5HT2B Receptor/HTR2B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain (lanes 1 and 5), rat brain (lanes 2 and 6), rat uterus (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma cell (lanes 4 and 8) lysates:1-4. Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035), (1:200).
5-8. Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody, preincubated with 5HT2B Receptor/HTR2B (extracellular) Blocking Peptide (#BLP-SR035).
Expression of 5-Hydroxytryptamine receptor 2B in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035) (1:400). A. 5-HT2B staining (red), appears in neurons of the Purkinje layer (vertical arrows) and in the molecular layer (horizontal arrow). B. Nuclear staining using DAPI as the counterstaining (blue). C. Merged image of panels A and B.
Properties
Sequence
- (C)KMSEQ STISEHILQK, corresponding to amino acid residues 6-20 of rat 5-Hydroxytryptamine receptor 2B (Accession P30994).
Accession (Uniprot) Number P30994
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
