Cat #: BLP-SR035
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)KMSEQ STISEHILQK, corresponding to amino acid residues 6-20 of rat 5-Hydroxytryptamine receptor 2B (Accession P30994).
Accession (Uniprot) Number P30994
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse brain (lanes 1 and 5), rat brain (lanes 2 and 6), rat uterus (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma cell (lanes 4 and 8) lysates:1-4. Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035), (1:200).
5-8. Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody, preincubated with 5HT2B Receptor/HTR2B (extracellular) Blocking Peptide (#BLP-SR035). - Mouse brain sections (1:400).
Last update: 02/01/2024