Overview
Type: Synthetic peptide
Form: Lyophilized Powder
5HT2C Receptor (HTR2C) Blocking Peptide (#BLP-SR034) is the original antigen used for immunization during Anti-5HT2C Receptor (HTR2C) Antibody (#ASR-034) generation. The blocking peptide binds and ‘blocks’ Anti-5HT2C Receptor (HTR2C) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1-2. Anti-5HT2C Receptor (HTR2C) Antibody (#ASR-034), (1:200).
3-4. Anti-5HT2C Receptor (HTR2C) Antibody, preincubated with 5HT2C Receptor (HTR2C) Blocking Peptide (BLP-SR034).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-5HT2C Receptor (HTR2C) Antibody (#ASR-034), (1:200).
2. Anti-5HT2C Receptor (HTR2C) Antibody, preincubated with 5HT2C Receptor (HTR2C) Blocking Peptide (BLP-SR034).
Expression of 5HT2C in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-5HT2C Receptor (HTR2C) Antibody (#ASR-034), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. 5HT2C immunoreactivity (green) appears in CA1 pyramidal cells (arrows). B. Pre-incubation of the antibody with 5HT2C Receptor (HTR2C) Blocking Peptide (BLP-SR034), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)RRQTLMLLRGHTEE, corresponding to amino acid residues 243 -256 of rat HTR2C (Accession P08909).
Accession (Uniprot) Number P08909
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
