Overview
Type: Synthetic peptide
Form: Lyophilized powder
5HT3A Receptor/HTR3A Blocking Peptide (#BLP-SR031) is the original antigen used for immunization during Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031) generation. The blocking peptide binds and ‘blocks’ Anti-5HT3A Receptor/HTR3A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031), (1:200).
3,4. Anti-5HT3A Receptor (HTR3A) Antibody, preincubated with 5HT3A Receptor/HTR3A Blocking Peptide (#BLP-SR031).
Expression of Serotonin receptor 3A in rat brainImmunohistochemical staining of rat parietal cortex using Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031), (1:400), (red). A. 5-HT3A staining appears in neuronal outlines (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Properties
Sequence
- (C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine receptor 3A (Accession P35563).
Accession (Uniprot) Number P35563
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
