Cat #: BLP-SR032
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B (Accession Q9JJ16).
Accession (Uniprot) Number Q9JJ16
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):1,2. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032), (1:200).
3,4. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody, preincubated with 5HT3B Receptor/HTR3B (extracellular) Blocking Peptide (#BLP-SR032). - Rat DRGs.
Last update: 02/01/2024