Overview
Type: Synthetic peptide
Form: Lyophilized powder
5HT5B Receptor/HTR5B (extracellular) Blocking Peptide (#BLP-SR040) is the original antigen used for immunization during Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody (#ASR-040) generation. The blocking peptide binds and ‘blocks’ Anti-5HT5B Receptor/HTR5B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat and mouse brain lysates:1,2. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody (#ASR-040), (1:400).
3,4. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody, preincubated with 5HT5B Receptor/HTR5B (extracellular) Blocking Peptide (#BLP-SR040).
Properties
Sequence
- CSDSPSSGRSMG, corresponding to amino acid residues 22-33 of rat 5-Hydroxytryptamine receptor 5B (Accession P35365).
Accession (Uniprot) Number P35365
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
