Overview
Type: Synthetic peptide
Form: Lyophilized Powder
ACE2 (extracellular) Blocking Peptide (#BLP-AC012) is the original antigen used for immunization during Anti-ACE2 (extracellular) Antibody (#AAC-012) generation. The blocking peptide binds and ‘blocks’ Anti-ACE2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat small intestine lysate (lanes 1 and 4), mouse lung lysate (lanes 2 and 5 ) and mouse kidney lysate (lanes 3 and 6):1-3. Anti-ACE2 (extracellular) Antibody (#AAC-012), (1:200).
4-6. Anti-ACE2 (extracellular) Antibody, preincubated with ACE2 (extracellular) Blocking Peptide (BLP-AC012).
Western blot analysis of human HepG2 hepatoma cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):1-2. Anti-ACE2 (extracellular) Antibody (#AAC-012), (1:200).
3-4. Anti-ACE2 (extracellular) Antibody, preincubated with ACE2 (extracellular) Blocking Peptide (BLP-AC012).
Expression of ACE2 in mouse thalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ACE2 (extracellular) Antibody (#AAC-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. ACE2 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with ACE2 (extracellular) Blocking Peptide (BLP-AC012), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EDYGDYWRGDYEVNGVD, corresponding to amino acid residues 197 - 213 of human ACE2 (Accession Q9BYF1).
Accession (Uniprot) Number Q9BYF1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
