Overview
Type: Synthetic peptide
Form: Lyophilized Powder
ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041) is the original antigen used for immunization during Anti-ACVRL1 (extracellular) Antibody (#ABR-041) generation. The blocking peptide binds and ‘blocks’ Anti-ACVRL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-ACVRL1 (extracellular) Antibody (#ABR-041), (1:200).
3-4. Anti-ACVRL1 (extracellular) Antibody, preincubated with ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041).
Western blot analysis of rat heart membranes:1. Anti-ACVRL1 (extracellular) Antibody (#ABR-041), (1:200).
2. Anti-ACVRL1 (extracellular) Antibody, preincubated with ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041).
Western blot analysis of Mouse endothelial MS1 cell line lysate (lanes 1 and 4), rat aortic endothelial cell line (RAEC) lysates (lanes 2 and 5) and mouse J774 macrophage cell line lysates (lanes 3 and 6):1-3. Anti-ACVRL1 (extracellular) Antibody (#ABR-041), (1:200).
4-6. Anti-ACVRL1 (extracellular) Antibody, preincubated with ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041).
Properties
Sequence
- Peptide CYRSF(S)NHNVSLMLE, corresponding to amino acid residues 89 - 103 of mouse ACVRL1 (Accession Q61288). Extracellular, N-terminus.
Accession (Uniprot) Number Q61288
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
