Overview
Type: Synthetic peptide
Form: Lyophilized Powder
ADAM10 (extracellular) Blocking Peptide (#BLP-NR156) is the original antigen used for immunization during Anti-ADAM10 (extracellular) Antibody (#ANR-156) generation. The blocking peptide binds and ‘blocks’ Anti-ADAM10 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-ADAM10 (extracellular) Antibody (#ANR-156), (1:500). 3-4. Anti-ADAM10 (extracellular) Antibody, preincubated with ADAM10 (extracellular) Blocking Peptide (BLP-NR156).
Western blot analysis of rat spleen lysate (lanes 1 and 3) and mouse spleen lysate (lanes 2 and 4):1-2. Anti-ADAM10 (extracellular) Antibody (#ANR-156), (1:500). 3-4. Anti-ADAM10 (extracellular) Antibody, preincubated with ADAM10 (extracellular) Blocking Peptide (BLP-NR156).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human K562 erythroleukemia cell line lysate (lanes 2 and 4):1-2. Anti-ADAM10 (extracellular) Antibody (#ANR-156), (1:200). 3-4. Anti-ADAM10 (extracellular) Antibody, preincubated with ADAM10 (extracellular) Blocking Peptide (BLP-NR156).
Expression of ADAM10 in mouse parietal cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ADAM10 (extracellular) Antibody (#ANR-156), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ADAM10 immunoreactivity (green) appears in neuronal profiles (horizontal arrows). B. Pre-incubation of the antibody with ADAM10 (extracellular) Blocking Peptide (BLP-NR156), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CTPGESKNLGQKENG, corresponding to amino acid residues 400 - 414 of mouse ADAM10 (Accession O35598).
Accession (Uniprot) Number O35598
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
