Overview
Type: Synthetic peptide
Form: Lyophilized powder
ADAM22 (extracellular) Blocking Peptide (#BLP-NR120) is the original antigen used for immunization during Anti-ADAM22 (extracellular) Antibody (#ANR-120) generation. The blocking peptide binds and ‘blocks’ Anti-ADAM22 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-ADAM22 (extracellular) Antibody (#ANR-120), (1:200).
3,4. Anti-ADAM22 (extracellular) Antibody, preincubated with ADAM22 (extracellular) Blocking Peptide (#BLP-NR120).
Expression of ADAM22 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-ADAM22 (extracellular) Antibody (#ANR-120), (1:200), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. ADAM22 staining (green) appears in Purkinje cells (vertical arrows) and in dendrites (horizontal arrows) in the molecular layer (Mol). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)HNDDAKTGITLSGNG, corresponding to amino acid residues 715-729 of mouse ADAM22 (Accession Q9R1V6).
Accession (Uniprot) Number Q9R1V6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
