Overview
Type: Synthetic peptide
Form: Lyophilized powder
Adenosine A1 Receptor Blocking Peptide (#BLP-AR006) is the original antigen used for immunization during Anti-Adenosine A1 Receptor Antibody (#AAR-006) generation. The blocking peptide binds and ‘blocks’ Anti-Adenosine A1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1, 3) and rat kidney (lanes 2, 4) lysates:1,2. Anti-Adenosine A1 Receptor Antibody (#AAR-006), (1:200).
3,4. Anti-Adenosine A1 Receptor Antibody, preincubated with Adenosine A1 Receptor Blocking Peptide (#BLP-AR006).
Expression of Adenosine A1 Receptor in rat cortexImmunohistochemical staining of rat cortex frozen section using Anti-Adenosine A1 Receptor Antibody (#AAR-006). A. A1AR (green) appears in neurons (triangles) and in astrocytes (arrows). B. Parvalbumin staining (red) appears in cortical interneurons. C. Confocal merge of images demonstrates the existence of A1AR in a subset of cortical interneurons and astrocytes.
Expression of Adenosine A1 Receptor in rat medial septumImmunohistochemical staining of rat medial septum frozen section using Anti-Adenosine A1 Receptor Antibody (#AAR-006). A. A1AR (green) appears in neurons (right pointing triangles). B. Parvalbumin staining (red) appears in medial septal neurons. C. Confocal merge of demonstrates expression of A1AR in a subset of medial septal neurons.
Properties
Sequence
- (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR (Accession P30542).
Accession (Uniprot) Number P30542
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
