Overview
Type: Synthetic peptide
Form: Lyophilized powder
Adenosine A2A Receptor (extracellular) Blocking Peptide (#BLP-AR008) is the original antigen used for immunization during Anti-Adenosine A2A Receptor (extracellular) Antibody (#AAR-008) generation. The blocking peptide binds and ‘blocks’ Anti-Adenosine A2A Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6):1-3. Anti-Adenosine A2A Receptor (extracellular) Antibody (#AAR-008), (1:200).
4-6. Anti-Adenosine A2A Receptor (extracellular) Antibody, preincubated with Adenosine A2A Receptor (extracellular) Blocking Peptide (#BLP-AR008).
Expression of Adenosine A2A Receptor in rat piriform cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Adenosine A2A Receptor (extracellular) Antibody (#AAR-008), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. Adenosine A2A Receptor immunoreactivity (red), appears in neurons (vertical arrows) and in glial cells in layers 1-2 (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SQKDENSTKT(S)GE, corresponding to amino acid residues 143-156 of mouse adenosine A2A receptor (Accession Q60613).
Accession (Uniprot) Number Q60613
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
