Overview
Cat #: BLP-AR002
Type: Synthetic peptide
Form: Lyophilized powder
Adenosine A2A Receptor Blocking Peptide (#BLP-AR002) is the original antigen used for immunization during Anti-Adenosine A2A Receptor Antibody (#AAR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Adenosine A2A Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat aortic endothelial cells (lanes 1, 3), rat brain (lanes 2, 4) and Jurkat (lanes 5, 6) lysates:1,2,5. Anti-Adenosine A2A Receptor Antibody (#AAR-002), (1:200).
3,4,6. Anti-Adenosine A2A Receptor Antibody, preincubated with Adenosine A2A Receptor Blocking Peptide (#BLP-AR002). - Expression of Adenosine A2A Receptor in mouse diagonal bandImmunohistochemical staining of mouse diagonal band using Anti-Adenosine A2A Receptor Antibody (#AAR-002). A2aR (green) appears in the broca of individual neurons (arrows) and in neuropil (asterisk). DAPI is used as the counterstain.
- Expression of Adenosine A2A Receptor in rat lungImmunohistochemical staining of paraffin emedded rat lung sections using Anti-Adenosine A2A Receptor Antibody (#AAR-002), (1:50). A2aR is expressed in the respiratory epithelium of the bronchioli (Br). Note that smooth muscle and endothelium in blood vessels are negative. Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse adenosine A2A receptor (Accession Q60613).
Accession (Uniprot) Number Q60613
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.